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November 1, 2002Journal of Biological ChemistryOpen Access

Loss of either G(z)alpha or G(i2)alpha impaired the ability of ADP and epinephrine to inhibit PGI(2)-stimulated adenylyl cyclase activity and caused a 40%-50% rise in basal cAMP levels, whereas loss of G(i3)alpha did not.

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Population

Platelets from mice lacking the alpha subunits of one or more of the three most abundantly expressed G…

Comparison

Genetic deletion of Galpha, Galpha, Galpha, or… vs Platelets from mice with different knockouts or…

Design

Preclinical

Authors

JYJing YangJWJie WuHJHong Jiang

Discussion

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Overview

Hypothesis-generating for Gi2/Gz-specific platelet cAMP control; human studies needed before clinical relevance.

Structured PICO

P
Population
Platelets from mice lacking the alpha subunits of one or more of the three most abundantly expressed G(i) family members (G(i2)alpha, G(z)alpha, G(i3)alpha) and mice lacking the PGI(2) receptor (IP)
I
Intervention
Genetic deletion of G(i2)alpha, G(z)alpha, G(i3)alpha, or IP receptor
C
Comparator
Platelets from mice with different knockouts or wild-type (implied)
O
Outcome
Platelet aggregation and cAMP formation in response to ADP, epinephrine, and PGI(2)surrogate

Platelet epinephrine and ADP receptors display strong preferences among Gi family members (Gi2 and Gz, but not Gi3) for regulating cAMP levels, highlighting specific non-redundant signaling pathways during platelet activation.

Cite This Study

Yang et al. (2002) studied this question.

synapsesocial.com/papers/6a702e27ac440176ef28d385https://doi.org/10.1074/jbc.m208519200
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Coordinated Signaling through Both G12/13 and Gi Pathways Is Sufficient to Activate GPIIb/IIIa in Human Platelets2002 · 123 citations
  2. 2Unresponsiveness of Platelets Lacking Both Gαq and Gα132004 · 70 citations
  3. 3Differential requirements for calcium and Src family kinases in platelet GPIIb/IIIa activation and thromboxane generation downstream of different G-protein pathways2004 · 44 citations
  4. 4RGS/Gi2α interactions modulate platelet accumulation and thrombus formation at sites of vascular injury2010 · 56 citations
  5. 5Differential requirements for platelet aggregation and inhibition of adenylate cyclase by epinephrine. Studies of a familial platelet alpha 2-adrenergic receptor defect1988 · 51 citations