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June 16, 2020BloodOpen Access

Deleting both RGS10 and RGS18 in mice reduces platelet count ~40% and exaggerates injury response.

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Why the study?

While RGS10 and RGS18 had each been studied individually, critical questions remained regarding the overall impact of RGS-mediated regulation in platelets.

Does the combined deletion of RGS10 and RGS18 affect platelet activation, production, and survival in mice?

Population

Mice missing RGS10, RGS18, or both (RGS10-/-18-/-)

Comparison

RGS10-/-18-/- mice vs individual knockouts (RGS10-/- and RGS18-/-)

Design

Preclinical animal study

Key result

Mice missing both RGS10 and RGS18 showed reduced platelet survival, a 40% decrease in platelet count, and an exaggerated response to injury compared to single knockouts.

Authors

DDDaniel DeHelianTranslational Therapeutics (United States)SGShuchi GuptaCollege of AccountingJWJie WuJinan University

Discussion

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Overview

Dual RGS10/RGS18 deletion impairs platelet survival in mice; hypothesis-generating for complementary regulation, with human relevance untested.

Structured PICO

Does the combined deletion of RGS10 and RGS18 affect platelet activation, production, and survival in mice?

P
Population
Mice missing RGS10, RGS18, or both (RGS10-/-18-/-)
I
Intervention
Genetic deletion of RGS10 and/or RGS18
C
Comparator
Individual knockouts (RGS10-/- or RGS18-/-) and wild-type controls
O
Outcome
Platelet survival, platelet count, and platelet reactivity (in vitro and in vivo)surrogate

RGS10 and RGS18 have complementary roles in regulating platelet reactivity, production, and survival in vivo.

Cite This Study

DeHelian et al. (2020) studied Platelet activation and survival. RGS10 and RGS18 double knockout vs. Single knockouts (RGS10-/-, RGS18-/-) and wild type was evaluated on Platelet count, survival, and reactivity. Mice missing both RGS10 and RGS18 showed reduced platelet survival, a 40% decrease in platelet count, and an exaggerated response to injury compared to single knockouts.

synapsesocial.com/papers/6a0ba9fa4f6759c6fca256b4https://doi.org/10.1182/blood.2019003251
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Modulating platelet reactivity through control of RGS18 availability2015 · 27 citations
  2. 2RGS/Gi2α interactions modulate platelet accumulation and thrombus formation at sites of vascular injury2010 · 56 citations
  3. 3RGS10 shapes the hemostatic response to injury through its differential effects on intracellular signaling by platelet agonists2018 · 21 citations
  4. 4GRK2 regulates ADP signaling in platelets via P2Y1 and P2Y122022 · 20 citations
  5. 5Regulator of G Protein Signaling Protein 6 (RGS6) Protects the Heart from Ischemic Injury2015