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April 1, 2015The FASEB Journal

RGS6-/- hearts had significantly larger infarcts than RGS6+/+ hearts (51 ± 2 % vs 34 ± 5 % area at risk), while deletion of RGS1, RGS2, RGS4, or RGS5 had no effect on infarct size.

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Why the study?

Does genetic deletion of specific RGS proteins influence myocardial sensitivity to ischemic injury in isolated mouse hearts?

Population

Hearts from RGS1-/-, RGS2-/-, RGS2-/-RGS4-/- double knockout, RGS5-/-, and RGS6-/- mice, and their…

Comparison

Genetic deletion of specific RGS proteins vs Wildtype (+/+) littermates

Design

Preclinical

Follow-up

2 hours reperfusion

Authors

BRBoyd R. RorabaughNMNathaniel W. MabeSSSarah L. Seeley

Discussion

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Overview

Does not inform clinical management of ischemia; hypothesis-generating for RGS6 cardioprotection in humans.

Structured PICO

Does genetic deletion of specific RGS proteins influence myocardial sensitivity to ischemic injury in isolated mouse hearts?

P
Population
Hearts from RGS1-/-, RGS2-/-, RGS2-/-RGS4-/- double knockout, RGS5-/-, and RGS6-/- mice, and their respective wildtype (+/+) littermates
I
Intervention
Genetic deletion of specific RGS proteins (RGS1, RGS2, RGS4, RGS5, RGS6)
C
Comparator
Wildtype (+/+) littermates
O
Outcome
Infarct size measured by triphenyltetrazolium chloride staining after 30 min ischemia and 2 hours reperfusion using the Langendorff isolated heart modelsurrogate

RGS6 protects the heart from ischemic injury, as its genetic deletion significantly increases infarct size in a mouse model.

Cite This Study

Rorabaugh et al. (2015) studied this question.

synapsesocial.com/papers/6a6f644b35aa2c282ce0c1eahttps://doi.org/10.1096/fasebj.29.1_supplement.1026.8
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Regulator of G Protein Signaling 6 Facilities Cardiac Hypertrophy by Activating Apoptosis Signal–Regulating Kinase 1–P38/c‐JUN N‐Terminal Kinase 1/2 Signaling2018 · 23 citations
  2. 2RGS6, a Modulator of Parasympathetic Activation in Heart2010 · 127 citations
  3. 3RGS6, but Not RGS4, Is the Dominant Regulator of G Protein Signaling (RGS) Modulator of the Parasympathetic Regulation of Mouse Heart Rate2013 · 36 citations
  4. 4The therapeutic potential of targeting cardiac RGS42023 · 2 citations
  5. 5RGS proteins in heart: brakes on the vagus2012 · 39 citations