Antibody binding to the S-2 region of myosin suppresses contractile force without inhibiting ATP hydrolysis, supporting the role of the S-2 hinge domain in muscle force generation.
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Highlights S-2 domain as force modulator independent of ATPase; leaves open therapeutic targeting in cardiomyopathies.
Polyclonal antibody directed against the subfragment-2 region of myosin was purified by affinity chromatography. Skinned muscle fibers that had been preincubated with antibody were able to sustain only 7% of the active isometric force generated by control fibers. The effect of antibody on force production could not be accounted for by inhibition of ATP turnover.
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Lovell et al. (1988) studied this question.
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