Inhibition of acanthamoeba actomyosin-II ATPase activity and mechanochemical function by specific monoclonal antibodies.
Population
Acanthamoeba myosin-II and cytoplasmic extracts
Comparison
Monoclonal and polyclonal antibodies that bind… vs Control antibodies that do not bind to myosin-II
Design
Preclinical
Authors
Discussion
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Member takes
Implication
No clinical implications for cardiovascular care; leaves open whether myosin-II tail domains regulate contractility in mammalian systems.
Structured PICO
P
Population
Acanthamoeba myosin-II and cytoplasmic extracts
I
Intervention
Monoclonal and polyclonal antibodies that bind to myosin-II
C
Comparator
Control antibodies that do not bind to myosin-II
O
Outcome
Inhibition of myosin ATPase activity, actomyosin ATPase activity, and contraction of cytoplasmic extractssurrogate
Specific monoclonal antibodies can inhibit Acanthamoeba actomyosin-II ATPase activity and gel contraction, revealing that structural domains in the myosin-II tail are involved in catalysis and energy transduction.
Cite This Study
Kiehart et al. (1984) studied this question.