Site-specific inhibition of myosin-mediated motility in vitro by monoclonal antibodies.
Population
Dictyostelium myosin in vitro model
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Should not yet inform clinical myosin-targeted therapies; leaves open the in vivo significance of these binding sites.
Structured PICO
P
Population
Dictyostelium myosin in vitro model
I
Intervention
Monoclonal antibodies directed against seven different sites on Dictyostelium myosin
O
Outcome
Inhibition of myosin movement in vitro using the Nitella-based myosin-mediated bead movement assaysurrogate
Specific monoclonal antibodies binding to the 18,000-dalton light chain or approximately 1,200 A from the head-tail junction of the rod completely inhibit Dictyostelium myosin movement in vitro.
Cite This Study
Flicker et al. (1985) studied this question.