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December 1, 1988The Journal of Cell Biology

Modulation of cellular morphology and locomotory activity by antibodies against myosin.

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Population

Chicken fibroblasts

Design

Preclinical

Authors

BHBernd HönerBielefeld UniversitySCSandra CitiUniversity of GenevaJKJohn Kendrick‐JonesHeart Failure / Cardiomyopathy

Discussion

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Implication

Preclinical findings only; leaves open myosin's role in cell motility for cardiovascular models.

Key Points

  • To investigate the role of nonmuscle myosin in microfilament organization, cell morphology, and locomotion in chicken fibroblasts.
  • Microinjected three distinct monoclonal antibodies targeting separate epitopes on the chicken brush border nonmuscle myosin heavy chain into chicken fibroblasts.
  • Assessed dose-dependent and reversible cellular responses, evaluating antibody-to-myosin molar ratios exceeding 3:1 on stress fiber integrity, lamellar expansion, and locomotion.
  • Microinjection of all three antibodies caused reversible, concentration-dependent breakdown of stress fibers and triggered extensive lamellae formation that drastically altered morphology.
  • Treated fibroblasts exhibited increased locomotory activity, with the most pronounced morphologic and dynamic alterations occurring at antibody-to-cellular myosin molar ratios greater than 3:1.

Structured PICO

P
Population
Chicken fibroblasts
I
Intervention
Injection of three monoclonal antibodies directed against chicken brush border myosin
O
Outcome
Cellular morphology (breakdown of stress fibers, development of lamellae) and locomotory activitysurrogate

Antibody-induced decrease in functional myosin triggers an increase in plasma membrane dynamics and locomotory activity in chicken fibroblasts.

Cite This Study

Höner et al. (1988) studied this question.

synapsesocial.com/papers/6a8c175a0ca85c7e33df2735https://doi.org/10.1083/jcb.107.6.2181
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