Population
Intact fibre bundles isolated from the flexor digitorum brevis of C57BL/6 mice
Comparison
Fatiguing the fibres with a series of tetanic… vs Control (unfatigued state)
Design
Preclinical
Key result
Fatigue-induced increases in inorganic phosphate at physiological temperature reduced tetanic force by approximately 20% primarily by depressing individual crossbridge force and accelerating crossbridge kinetics.
Authors
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Provides mechanistic insight into muscle fatigue without immediate clinical application; leaves open whether targeting phosphate kinetics could yield therapeutic.
At physiological temperature, the increase of inorganic phosphate during early fatigue reduces tetanic force mainly by depressing individual crossbridge force and accelerating crossbridge kinetics.
Nocella et al. (2017) studied Healthy (mouse muscle fibers) (n=13). Fatigue induced by a series of tetanic contractions vs. Control (unfatigued) contractions was evaluated on Tetanic force at the 10th tetanus. Fatigue-induced increases in inorganic phosphate at physiological temperature reduced tetanic force by approximately 20% primarily by depressing individual crossbridge force and accelerating crossbridge kinetics.