Key result
Addition of 30 mM inorganic phosphate significantly increased actin translocation velocity at pH 6.8 and 6.5, causing a doubling of velocity at pH 6.5, with effects being highly ATP-dependent.
Population
Skeletal muscle myosin and actin in an in vitro motility assay
Comparison
Addition of inorganic phosphate under varying pH… vs Control conditions without added Pi or at…
Design
Preclinical
Authors
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Does not warrant changes in clinical management of myocardial acidosis; extends myosin kinetics data but requires in vivo cardiac validation.
Inorganic phosphate differentially affects myosin function, enhancing velocity when rebinding to the ADP-bound state and slowing velocity when binding to the rigor state.
Debold et al. (2011) studied this question. Inorganic phosphate (P(i)) was evaluated on Actin translocation velocity (V(actin)). Addition of 30 mM inorganic phosphate significantly increased actin translocation velocity at pH 6.8 and 6.5, causing a doubling of velocity at pH 6.5, with effects being highly ATP-dependent.
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