Key result
Rapid changes in muscle fibre length and load revealed that myosin heads tilt during the elastic response and the working stroke, with the average working stroke measuring 12 nm at low load.
Population
Intact single muscle fibres (frog skeletal muscle)
Comparison
Rapid changes in fibre length during active… vs Isometric contraction
Design
Preclinical
Authors
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Advances molecular models of contraction; leaves open validation in human cardiac muscle.
X-ray diffraction of single muscle fibres demonstrates that the myosin working stroke involves tilting of the light-chain domain, providing structural evidence for the molecular mechanism of muscle contraction.
Holmes et al. (2004) studied Muscle contraction (basic science). Rapid changes in fibre length or load vs. Isometric contraction was evaluated on Changes in the intensities and interference fine structure of axial X-ray reflections (M3). Rapid changes in muscle fibre length and load revealed that myosin heads tilt during the elastic response and the working stroke, with the average working stroke measuring 12 nm at low load.
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