January 1, 1993Nucleic Acids ResearchOpen Access

Iron regulatory factor expressed from recombinant baculovirus: conversion between the RNA-binding apoprotein and Fe-S cluster containing aconitase

View Full Paper

Ask AI

Bookmark

Population

Insect cells expressing human iron regulatory factor (IRF) from recombinant baculovirus

Comparison

High iron medium or in vitro Fe-S… vs Absence of iron (apoprotein synthesis)

Design

Preclinical

Authors

Discussion

Reciprocal IRF regulation by Fe-S cluster is iron-dependent; leaves open whether targeting alters human iron homeostasis.

Population

Insect cells expressing human iron regulatory factor (IRF) from recombinant baculovirus

Intervention

High iron medium or in vitro Fe-S cluster-generating conditions

Comparator

Absence of iron (apoprotein synthesis)

Outcome

IRE-binding capacity and aconitase activitysurrogate

The RNA-binding and aconitase activities of human iron regulatory factor are mutually exclusive and controlled by the formation of an Fe-S cluster.

Emery-Goodman et al. (1993) studied this question.

View Full Paper

Ask AI

Bookmark