Answers are grounded in this paper and cite their sources.
A method was developed for analysis of cholesterol in whole milk powder and commercial liquid milk products. High performance liquid chromatography employed a nonaqueous reversed phase chromatographic system. Sample preparation was by saponification with methanolic potassium hydroxide followed by extraction with ether. Potential interfering substances were removed prior to injection by a simple clean-up procedure using a silica gel Sep-pakTM. The chromatographic system included a carbon-18 column with a hexane/isopropanol mobile phase and detection at 205 nm. Method validation and recovery studies using carbon-14 labeled cholesterol indicated excellent performance.
No takes yet. Share an insight, caveat, or question.
Hurst et al. (1983) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: