The exterior surface of the chicken erythrocyte

The exterior surface of mature chicken erythrocytes has been labeled with a cationic membrane impermeable reagent, rho-nitrophenyl-N,N,N-trimethy[125I]iodotyrosinate. This reagent forms stable covalent bonds with the amino groups of exposed protein and lipid companents. Two major protein components with subunit molecular weights of 100,000 and 55,000 are found on the outer surface of mature chicken erythrocytes. Both of the labeled components coincide were periodic acid-Schiff base-stained areas on sodium dodecyl sulfate polyacrylamide gel electrophoresis indicating that they may be glycoproteins.