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January 1, 1989Nucleic Acids ResearchOpen Access

Oligonudeotide-targeted degradation of U1 and U2 snRNAs reveals differential interactions of simian virus 40 pre-mRNAs with snRNPs

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Structured PICO

P

Population

Xenopus laevis oocytes and HeLa splicing extracts

I

Intervention

Microinjection of oligonucleotides complementary to regions of U1 or U2 RNAs

O

Outcome

Cleavage of corresponding snRNA and accumulation of spliced SV40 mRNA

Different pre-mRNAs vary in their requirements for snRNPs during splicing.

Discussion

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Implication

Oligonucleotide splicing effects in viral models are hypothesis-generating; leaves open differential snRNP requirements across endogenous pre-mRNAs.

Cite This Study

Pan et al. (1989) studied this question.

synapsesocial.com/papers/6a86f90de0e6abca40d3c10f https://doi.org/10.1093/nar/17.16.6553

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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Factors Influencing Alternative Splice Site Utilization In VivoOriginal Research · 1987 · 48 citations
2Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytesIn Vitro Study · 1968 · 306 citations
3pBR322 DNA inhibits simian virus 40 gene expression inXenopus laevisoocytesIn Vitro Study · 1987 · 19 citations
4Linear DNA Does Not Form Chromatin Containing Regularly Spaced NucleosomesOriginal Research · 1982 · 28 citations
5Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. brucei: identification of the U2, U4, and U6 RNA analogs.Original Research · 1989 · 119 citations