What question did this study set out to answer?

Investigate whether adipose tissue exosome-derived miR-4472 impairs skeletal muscle glucose uptake and promotes insulin resistance through the downregulation of MEF2D.

May 8, 2025Journal of Diabetes InvestigationOpen Access

Adipocyte exosome miR‐4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D

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Investigate whether adipose tissue exosome-derived miR-4472 impairs skeletal muscle glucose uptake and promotes insulin resistance through the downregulation of MEF2D.
Assessed cellular glucose uptake and insulin signaling in vitro using cultured C2C12 muscle cells and 3T3-L1 adipocytes.
Evaluated insulin sensitivity and gene expression in vivo using diet-induced obesity mouse models and adipose tissue-specific Dicer knockout (AT-Dicer KO) mice.
Administered serum exosomes isolated from normal-weight and obese human individuals to mice alongside exosome and miR-4472 inhibitors.
Circulating miR-4472 correlated positively with BMI, waist and hip circumference, and fasting plasma glucose, with elevated secretion in adipose-derived exosomes during obesity.
Exosomal miR-4472 directly suppressed MEF2D and GLUT4 expression in skeletal muscle cells, significantly decreasing glucose consumption and cellular uptake.
Inhibition of exosomes or miR-4472 reversed MEF2D/GLUT4 downregulation, restored skeletal muscle glucose transport, and alleviated insulin resistance in obese mice.

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Implication

Translational study demonstrates that adipocyte exosomal miR-4472 impairs muscle glucose uptake via MEF2D repression in mice, highlighting a novel target for obesity-linked insulin resistance.

Cite This Study

Sun et al. (2025) studied this question.

synapsesocial.com/papers/6a86fc41a131ca67071554ba https://doi.org/10.1111/jdi.70054

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Implication

Translational study demonstrates that adipocyte exosomal miR-4472 impairs muscle glucose uptake via MEF2D repression in mice, highlighting a novel target for obesity-linked insulin resistance.

Adipocyte exosome miR‐4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D

Key Points

Discussion

Implication

Cite This Study

Adipocyte exosome miR‐4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D

Key Points

Discussion

Implication

Cite This Study

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