What question did this study set out to answer?

To examine renal vascular reactivity to angiotensin II in the nonclipped kidney of two-kidney, one-clip hypertensive rats compared to normotensive controls.

March 23, 2005AJP Renal Physiology

The renal vascular response to ANG II injection is reduced in the nonclipped kidney of two-kidney, one-clip hypertension

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Why the study?

Does the nonclipped kidney in 2K1C hypertensive rats have an altered renal blood flow response to ANG II compared to normotensive controls?

Population

Two-kidney, one-clip (2K1C) hypertensive rats (6 weeks after clipping) and normotensive control rats

Comparison

Intra-renal artery injection of 2.5 ng… vs Normotensive control rats receiving the same…

Design

Preclinical

Follow-up

6 weeks after clipping

Authors

LBLiliana Monica BivolUniversity of Bergen ØVØyvind VågnesUniversity of North Carolina at Chapel Hill BIBjarne Magnus IversenHaukeland University Hospital

Discussion

Member takes

Implication

No direct clinical implications; hypothesis-generating for AT1 receptor downregulation in 2K1C renovascular models.

Key Points

To examine renal vascular reactivity to angiotensin II in the nonclipped kidney of two-kidney, one-clip hypertensive rats compared to normotensive controls.
Measured renal blood flow changes via transit-time flowmetry in response to intra-arterial injections of 2.5 ng angiotensin II and 5 ng arginine-vasopressin 6 weeks post-clipping.
Evaluated vascular responses before and after administration of indomethacin and candesartan, alongside plasma renin activity measurements.
Quantified AT1A and AT1B mRNA expression and AT1 receptor protein abundance in renal resistance vessels using Western blotting.
Systolic blood pressure was significantly elevated in 2K1C rats compared to controls (183 ± 4 vs. 113 ± 1 mmHg, P < 0.001), accompanied by higher plasma renin activity (P < 0.05).
Angiotensin II injection reduced renal blood flow by 10 ± 2% in the nonclipped kidney versus 24 ± 3% in controls (P < 0.001), requiring 30 µg/kg candesartan for full inhibition versus 100 µg/kg in controls (P < 0.001).
Indomethacin increased the blood flow reduction from 10 ± 2% to 20 ± 3% (P < 0.02) in 2K1C rats and from 24 ± 3% to 34 ± 6% (P < 0.01) in controls, despite unchanged total AT1 receptor mRNA and protein levels.

Structured PICO

Does the nonclipped kidney in 2K1C hypertensive rats have an altered renal blood flow response to ANG II compared to normotensive controls?

Population

Two-kidney, one-clip (2K1C) hypertensive rats (6 weeks after clipping) and normotensive control rats

Intervention

Intra-renal artery injection of 2.5 ng angiotensin II (ANG II), before and after indomethacin and candesartan treatment

Comparator

Normotensive control rats receiving the same injections

Outcome

Renal blood flow (RBF) response to ANG IIsurrogate

Despite unchanged total AT1 receptor levels, functional AT1 receptors are downregulated in the nonclipped kidney of 2K1C hypertensive rats, leading to a reduced renal blood flow response to ANG II.

Cite This Study

Bivol et al. (2005) studied this question.

synapsesocial.com/papers/6a87c47cbc6dec9ec226275c https://doi.org/10.1152/ajprenal.00319.2004

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The renal vascular response to ANG II injection is reduced in the nonclipped kidney of two-kidney, one-clip hypertension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The renal vascular response to ANG II injection is reduced in the nonclipped kidney of two-kidney, one-clip hypertension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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