Key Points
- To characterize glomerular and preglomerular vascular angiotensin II receptors during the acute phase of nonrenin-dependent hypertension and evaluate their regulation following renin-angiotensin system blockade.
- One-kidney, one clip hypertension was induced in male Sprague-Dawley rats using a 0.2 mm silver clip on the left renal artery alongside contralateral nephrectomy, with evaluations conducted at 1, 2, or 4 weeks.
- Purified glomerular and preglomerular vascular membranes were examined via competitive binding assays using losartan and PD 123319, including after two weeks of treatment with either captopril or TCV-116.
- Only angiotensin II type 1 (AT1) receptors were identified across tissues, showing significantly higher vascular AT1 receptor density at 1 and 2 weeks in hypertensive rats without significant changes in glomerular receptor density.
- Renin-angiotensin system blockade caused significant differential changes in glomerular AT1 receptor density—increasing with captopril and decreasing with TCV-116—while vascular AT1 receptor density remained unaffected.
Structured PICO
PPopulationMale Sprague-Dawley rats with one-kidney, one clip hypertension
IInterventionCaptopril (angiotensin converting enzyme inhibitor) or TCV-116 (angiotensin II receptor antagonist) for 2 weeks
CComparatorUntreated one-kidney, one clip hypertensive rats and age-matched uninephrectomized controls
OOutcomeGlomerular and preglomerular vascular angiotensin II receptor densitysurrogate
In a rat model of one-kidney, one clip hypertension, glomerular but not vascular angiotensin II receptors are differentially regulated depending on the type of renin-angiotensin system blockade.