February 1, 1992Journal of VirologyOpen Access

Cellular proteins bind to the 3' end of Sindbis virus minus-strand RNA

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Population

Extracts from Sindbis virus-infected or uninfected cells

Comparison

Short oligonucleotide probes representing the… vs Wild-type vs mutant probe

Design

Preclinical

Authors

NPNathalie PardigonInstitut Pasteur JSJames H. StraussCalifornia Institute of Technology

Discussion

Member takes

Implication

Transient host-RNA binding may be essential for alphavirus replication; leaves open whether these interactions represent antiviral targets.

Structured PICO

Population

Extracts from Sindbis virus-infected or uninfected cells

Intervention

Short oligonucleotide probes (62 or 132 nucleotides) representing the 3'-terminal sequence of the minus strand (wild-type and mutant)

Comparator

Wild-type vs mutant probe

Outcome

Binding of cellular proteins to the RNA probessurrogate

Cellular proteins bind specifically to the 3' end of Sindbis virus minus-strand RNA, and overly tight binding by a lethal mutant suggests that transient binding is required for viral replication.

Cite This Study

Pardigon et al. (1992) studied this question.

synapsesocial.com/papers/6a87d30f74703a9a15e962f3 https://doi.org/10.1128/jvi.66.2.1007-1015.1992

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Implication

Transient host-RNA binding may be essential for alphavirus replication; leaves open whether these interactions represent antiviral targets.

Cellular proteins bind to the 3' end of Sindbis virus minus-strand RNA

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Cellular proteins bind to the 3' end of Sindbis virus minus-strand RNA

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider