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December 1, 1989Journal of VirologyOpen Access

In vitro construction of poliovirus defective interfering particles

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Population

HeLa S3 monolayer cells

Design

Preclinical

Authors

KHKimiko Hagino‐YamagishiTokyo Metropolitan Institute of Medical ScienceANAkio NomotoTokyo Medical and Dental University

Discussion

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Implication

Provides platform for poliovirus DI studies; leaves open in vivo translation and clinical applications.

Structured PICO

P
Population
HeLa S3 monolayer cells
I
Intervention
Transfection with synthesized RNA from cloned poliovirus cDNA (pSM1(T7)1, pSM1(T7)1a, pSM1(T7)1b) and superinfection with standard virus as a helper
O
Outcome
Construction and replication of poliovirus defective interfering (DI) particles

The study successfully constructed poliovirus defective interfering particles in vitro, demonstrating that deletion RNAs must be active replicons to serve as genomes for DI particles.

Cite This Study

Hagino‐Yamagishi et al. (1989) studied this question.

synapsesocial.com/papers/6a87d4049842aaac2ffb129ahttps://doi.org/10.1128/jvi.63.12.5386-5392.1989
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Construction and characterization of poliovirus subgenomic replicons1988 · 120 citations
  2. 2Defective Interfering Particles of Poliovirus IV. Mechanisms of Enrichment1973 · 29 citations
  3. 3Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.1986 · 343 citations
  4. 4Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.1981 · 555 citations
  5. 5Genetic analysis of the attenuation phenotype of poliovirus type 11986 · 191 citations