Defective Interfering Particles of Poliovirus IV. Mechanisms of Enrichment
Population
HeLa cells infected by mixtures of standard poliovirus and defective, interfering (DI) poliovirus particles
Comparison
Various manipulations including radioactive… vs Standard infection conditions
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
These mechanisms should not yet influence clinical virology practice; leaves open targeted modulation of DI particles in research settings.
Structured PICO
P
Population
HeLa cells infected by mixtures of standard poliovirus and defective, interfering (DI) poliovirus particles
I
Intervention
Various manipulations including radioactive uridine addition, temperature shift from 37 to 31 C, continuous infection at 31 C, and cycloheximide treatment
C
Comparator
Standard infection conditions (e.g., 37 C, no cycloheximide)
O
Outcome
Ratio of DI particles in the progeny (enrichment)surrogate
The enrichment of defective interfering poliovirus particles in HeLa cells is driven by preferential RNA synthesis and encapsidation, which can be modulated by temperature and cycloheximide.
Cite This Study
Cole et al. (1973) studied this question.