Poliovirus type 1 rapidly dominates vesicular stomatitis virus (VSV) production in doubly infected HeLa cells by shutting off VSV-specific protein synthesis at the translation level.
Poliovirus type 1 interferes with VSV virion production in doubly infected HeLa cells primarily at the level of translation of viral messenger RNA.
In doubly infected HeLa cells, poliovirus type 1 rapidly and completely dominates vesicular stomatitis virus (VSV) plaque-forming unit production. Poliovirus type 1 shuts off incorporation of amino acids into VSV-specific proteins within 2 hr after superinfection of cells already committed to massive synthesis of VSV proteins. However, poliovirus type 1 appears to have little, if any, direct effect upon incorporation of uridine into VSV-directed ribonucleic acid (RNA) synthesis. Poliovirus apparently interferes with VSV virion production only at the level of translation of viral messenger RNA, although it interferes with host cell macromolecular syntheses at the levels of translation and transcription.
Doyle et al. (1972) studied Doubly infected HeLa cells. Poliovirus type 1 superinfection vs. Vesicular stomatitis virus (VSV) alone was evaluated on VSV plaque-forming unit production and protein/RNA synthesis. Poliovirus type 1 rapidly dominates vesicular stomatitis virus (VSV) production in doubly infected HeLa cells by shutting off VSV-specific protein synthesis at the translation level.
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