What question did this study set out to answer?

To determine whether alterations in hydrodynamic forces, including fluid flow rates and vascular pressures, affect prostacyclin production in intact lungs and isolated endothelial cells.

August 1, 1984Journal of Applied Physiology

Altering hydrodynamic variables influences PGI2 production by isolated lungs and endothelial cells

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Why the study?

Do hydrodynamic variables influence prostacyclin production in isolated lungs and endothelial cells?

Population

Isolated lungs and cultured endothelial cell monolayers

Comparison

Alterations in hydrodynamic variables vs Control flow, pressure, and shear conditions

Design

Preclinical

Authors

AGAlbertus van GrondelleNorthwestern University GWG. Scott WorthenNational Jewish Health DEDorette Z. EllisUniversity of North Texas

Discussion

Member takes

Implication

Edema dominates prostacyclin release over flow or pressure changes in isolated lungs; leaves open relevance to intact or human circulation.

Key Points

To determine whether alterations in hydrodynamic forces, including fluid flow rates and vascular pressures, affect prostacyclin production in intact lungs and isolated endothelial cells.
Perfused isolated lungs with salt solutions containing Ficoll or albumin under varying flow rates, pressure pulsatilities, and elevated venous pressures (up to 7.5 mmHg), with and without indomethacin.
Subjected cultured endothelial cell monolayers to defined shear rates and quantified prostacyclin accumulation in the supernatant.
Prostacyclin production increased proportionally with increases in perfusion flow and pressure drop, but was abolished in the presence of indomethacin and unaffected by pulsatility changes.
Increasing venous pressure from 2.1 to 4.8 mmHg had no effect, but raising it to 7.5 mmHg triggered edema and caused a massive increase in prostacyclin production.
Cultured endothelial cells rapidly reached peak prostacyclin release upon exposure to shear stress, exhibiting no further increase at higher shear rates.

Structured PICO

Do hydrodynamic variables influence prostacyclin production in isolated lungs and endothelial cells?

Population

Isolated lungs and cultured endothelial cell monolayers

Intervention

Alterations in hydrodynamic variables (changes in flow, pressure pulsatility, venous pressure, and defined shear rates)

Comparator

Control flow, pressure, and shear conditions

Outcome

Prostacyclin (PGI2) productionsurrogate

Hydrodynamic disturbances stimulate arachidonate metabolism and prostacyclin production in endothelial cells and isolated lungs, though edema formation causes a much larger increase.

Cite This Study

Grondelle et al. (1984) studied this question.

synapsesocial.com/papers/6a87e04b19ee2aac066c8e50 https://doi.org/10.1152/jappl.1984.57.2.388

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Implication

Edema dominates prostacyclin release over flow or pressure changes in isolated lungs; leaves open relevance to intact or human circulation.

Altering hydrodynamic variables influences PGI2 production by isolated lungs and endothelial cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Altering hydrodynamic variables influences PGI2 production by isolated lungs and endothelial cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider