Effects of prostaglandins, cAMP, and changes in cytosolic calcium on platelet aggregation induced by a thromboxane A2 mimic (U46619)

Human platelets (in vitro model)

U46619 (1 x 10(-7) M) with or without pretreatment with EGTA (3 x 10(-3) M), verapamil (5 x 10(-4) M), 8-(diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (1 x 10(-3) M), PGI2 (5 x 10(-8) M), PGD2 (5 x 10(-8) M), PGE1 (5 x 10(-8) M), PGF2 alpha (1 x 10(-5) M), dibutyryl cAMP (5 x 10(-3) M), or forskolin (1 x 10(-6) M)

U46619 alone without pretreatment

Cytosolic Ca2+ concentration and platelet aggregationsurrogate