Key result
KB-R7943 significantly inhibited the mitochondrial Ca2+ uniporter but had no effect on the mitochondrial Na+-Ca2+ exchanger in cardiomyocyte-derived H9c2 cells.
Why the study?
The study was conducted to examine the effect of KB-R7943, an inhibitor of the plasmalemmal Na+-Ca2+ exchanger, on mitochondrial Ca2+ transporters.
Population
Membrane-permeabilized cardiomyocyte-derived H9c2 cells expressing yellow cameleon 3.1 in the mitochondria
Design
In vitro laboratory study
Authors
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KB-R7943's selective mitochondrial effects merit exploration in cardioprotection models; leaves open translation to clinical ischemia-reperfusion injury.
p-value: p=<0.05
KB-R7943 inhibits the mitochondrial Ca2+ uniporter but not the mitochondrial Na+–Ca2+ exchanger in cardiomyocyte-derived H9c2 cells, which may contribute to its protective effects against ischemia-reperfusion injury.
Namekata et al. (2020) studied this question. KB-R7943 vs. Untreated control was evaluated on Mitochondrial Ca2+ uniporter and Na+-Ca2+ exchanger activity measured by fluorescence ratio (p=<0.05). KB-R7943 significantly inhibited the mitochondrial Ca2+ uniporter but had no effect on the mitochondrial Na+-Ca2+ exchanger in cardiomyocyte-derived H9c2 cells.
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