What question did this study set out to answer?

To determine whether cell swelling induced by hypotonic stress or solute transport activates basolateral potassium conductance in Necturus intestinal epithelium.

June 1, 1984Proceedings of the National Academy of SciencesOpen Access

Cell swelling increases a barium-inhibitable potassium conductance in the basolateral membrane of Necturus small intestine.

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Authors

KLK. R. LauUniversity of Manchester RHRandall L. HudsonUniversity at Buffalo, State University of New York SSStanley G. SchultzTexas Medical Center

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Implication

Electrophysiological study demonstrates that cell swelling increases basolateral potassium conductance in Necturus intestine, suggesting volume regulation drives repolarization during nutrient uptake.

Key Points

To determine whether cell swelling induced by hypotonic stress or solute transport activates basolateral potassium conductance in Necturus intestinal epithelium.
Measured mucosal membrane potential difference and mucosal-to-basolateral membrane resistance ratios in isolated Necturus small intestine.
Assessed electrophysiological responses to 12% hypotonic solutions and 15 mM mucosal galactose with or without 5 mM serosal barium and metabolic inhibitors.
Exposure to a 12% hypotonic solution induced mucosal membrane hyperpolarization and increased the membrane resistance ratio, effects that were abolished by metabolic inhibitors or 5 mM barium.
Addition of 15 mM mucosal galactose during serosal barium exposure caused initial depolarization without subsequent repolarization; repolarization and resistance increases resumed only after barium removal.
The basolateral membrane features a barium-inhibitable, metabolism-dependent potassium conductance that activates in response to hypotonic or solute-driven cell swelling.

Cite This Study

Lau et al. (1984) studied this question.

synapsesocial.com/papers/6a887485aadaab60564fd356 https://doi.org/10.1073/pnas.81.11.3591

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Cell swelling increases a barium-inhibitable potassium conductance in the basolateral membrane of Necturus small intestine.

Discussion

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Implication

Key Points

Cite This Study

Cell swelling increases a barium-inhibitable potassium conductance in the basolateral membrane of Necturus small intestine.

Discussion

Member takes

Implication

Key Points

Cite This Study

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