What question did this study set out to answer?

To investigate the influence of bovine albumin on myocardial capillary permeability across a range of hydrophilic solutes of varying molecular sizes in isolated perfused rabbit hearts.

October 1, 1981The Journal of PhysiologyOpen Access

Alterations of myocardial capillary permeability by albumin in the isolated, perfused rabbit heart.

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Why the study?

Does bovine albumin alter myocardial capillary permeability to hydrophilic solutes in isolated rabbit hearts?

Population

Isolated rabbit hearts

Comparison

Perfusion with Krebs-Ringer solution containing… vs Perfusion with albumin-free Krebs-Ringer solution

Design

Preclinical

Authors

GMGiovanni E. MannKing's College London

Discussion

Member takes

Implication

Albumin may stabilize capillary barrier function in isolated rabbit hearts; leaves open translation to human myocardial permeability or clinical relevance.

Key Points

To investigate the influence of bovine albumin on myocardial capillary permeability across a range of hydrophilic solutes of varying molecular sizes in isolated perfused rabbit hearts.
Perfused isolated rabbit hearts at constant flow rates between 0.2 and 4.7 ml·min⁻¹·g⁻¹ using the single-passage, multiple-tracer dilution technique.
Measured permeability-surface area products for 22Na, 51Cr-EDTA (mol. wt. 357), [57Co]cyanocobalamin (mol. wt. 1353), and 125I-labelled insulin (mol. wt. ~6000) in Krebs-Ringer solution with (10 g·l⁻¹) or without bovine albumin.
In the presence of albumin (10 g·l⁻¹), capillary permeability coefficients were 10.5, 3.5, 2.1, and 0.21 × 10⁻⁵ cm·s⁻¹ for 22Na, 51Cr-EDTA, [57Co]cyanocobalamin, and 125I-labelled insulin (diffusion-limited value 0.06 ± 0.02 ml·min⁻¹·g⁻¹, n=10), respectively.
Switching from an albumin-containing perfusate to an albumin-free solution led to a significant increase in permeability-surface area products for 22Na, 51Cr-EDTA, and [57Co]cyanocobalamin.

Structured PICO

Does bovine albumin alter myocardial capillary permeability to hydrophilic solutes in isolated rabbit hearts?

Population

Isolated rabbit hearts

Intervention

Perfusion with Krebs-Ringer solution containing bovine albumin (10 g/L)

Comparator

Perfusion with albumin-free Krebs-Ringer solution

Outcome

Capillary permeability-surface area products for 22Na, 51Cr-EDTA, [57Co]cyanocobalamin and 125I-labelled insulinsurrogate

Bovine albumin reduces the permeability of myocardial capillaries to hydrophilic solutes of varying molecular sizes, likely through interaction with transcapillary exchange pathways.

Cite This Study

Giovanni E. Mann (1981) studied this question.

synapsesocial.com/papers/6a887bb0b0d802598bf4f79a https://doi.org/10.1113/jphysiol.1981.sp013910

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Implication

Albumin may stabilize capillary barrier function in isolated rabbit hearts; leaves open translation to human myocardial permeability or clinical relevance.

Alterations of myocardial capillary permeability by albumin in the isolated, perfused rabbit heart.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Alterations of myocardial capillary permeability by albumin in the isolated, perfused rabbit heart.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider