What question did this study set out to answer?

To construct a comprehensive two-dimensional proteome map and database for Haemophilus influenzae, representing its sequenced genome of approximately 1,742 open reading frames.

January 1, 2000Electrophoresis

Two-dimensional map of the proteome ofHaemophilus influenzae

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Authors

HLHanno LangenRoche (Switzerland)BTBéla TakácsRoche (Switzerland)SEStefan EversUniversity of Münster

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Implication

Proteomic analysis reveals 502 distinct proteins in Haemophilus influenzae, highlighting comprehensive reference mapping for sequenced bacterial genomes.

Key Points

To construct a comprehensive two-dimensional proteome map and database for Haemophilus influenzae, representing its sequenced genome of approximately 1,742 open reading frames.
Analyzed soluble and envelope-bound protein fractions using two-dimensional electrophoresis across immobilized pH gradients, varying acrylamide concentrations, and ionic detergent systems.
Enriched low-copy-number proteins through extraction protocols and chromatographic fractionation, including heparin chromatography, chromatofocusing, and hydrophobic interaction chromatography.
Identified resolved protein spots using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and amino acid composition analysis.
Resolved and identified 502 distinct proteins from the predicted 1,742 open reading frames of Haemophilus influenzae.
Established one of the largest reference two-dimensional proteome databases for a medically important bacterium across soluble and membrane-bound fractions.

Cite This Study

Langen et al. (2000) studied this question.

synapsesocial.com/papers/6a88edc246f665d09875fe9d https://doi.org/10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4

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Implication

Proteomic analysis reveals 502 distinct proteins in Haemophilus influenzae, highlighting comprehensive reference mapping for sequenced bacterial genomes.

Two-dimensional map of the proteome ofHaemophilus influenzae

Discussion

Member takes

Implication

Key Points

Cite This Study

Two-dimensional map of the proteome ofHaemophilus influenzae

Discussion

Member takes

Implication

Key Points

Cite This Study

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