December 1, 1997British Journal of PharmacologyOpen Access

Evidence of an important and direct role for protein kinase C in agonist‐induced phosphorylation leading to desensitization of the angiotensin AT1A receptor

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Population

Human embryonic kidney cells stably expressing a recombinant AT1A receptor with a cleavable6 affinity tag

Comparison

Angiotensin II at low and high concentrations… vs Control conditions

Design

Preclinical

Authors

ABAnthony J. BalmforthGeneral / Preventive / Lipids FSFiona H. ShepherdUniversity of Leeds PWPhilip WarburtonUniversity of Plymouth

Discussion

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Implication

Does not yet alter clinical angiotensin management; extends preclinical understanding of concentration-dependent AT1A desensitization.

Structured PICO

Population

Human embryonic kidney cells stably expressing a recombinant AT1A receptor with a cleavable (His)6 affinity tag

Intervention

Angiotensin II (AII) at low (1 nM) and high (100 nM) concentrations, with or without preincubation with Ro-31-8220 (a PKC specific inhibitor)

Comparator

Control conditions (no inhibitor or different AII concentrations)

Outcome

Agonist-induced receptor phosphorylation and desensitizationsurrogate

Protein kinase C plays a direct and physiologically relevant role in the phosphorylation and desensitization of the angiotensin AT1A receptor at low, physiological concentrations of angiotensin II.

Cite This Study

Balmforth et al. (1997) studied this question.

synapsesocial.com/papers/6a89087dc5b3adf02218b495 https://doi.org/10.1038/sj.bjp.0701522

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Evidence of an important and direct role for protein kinase C in agonist‐induced phosphorylation leading to desensitization of the angiotensin AT1A receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Evidence of an important and direct role for protein kinase C in agonist‐induced phosphorylation leading to desensitization of the angiotensin AT1A receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider