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In these studies, the errors resulting from undisturbed creaming (3, 7, 10, 13) were minimized by inversion of tubes at regular intervals until incipient reduction was noted.The wider ratio (1: 2) reported by Collins et al. (1) may be due to their incubating tubes undisturbed, or to end-point differences.Reduction time for resazurin represents the time taken to change to a full pink color; for methylene blue, the time required to decolorize at least 90 per cent of the column of milk.295
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Johns et al. (1940) studied this question.
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