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April 1, 1989Journal of VirologyOpen Access

Genetic dissection of the transactivating domain of the E1a 289R protein of adenovirus type 2

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Population

Adenovirus type 2 E1a gene (pm975 variant) and infected cells

Comparison

Linker-scanning, deletion, and frameshift… vs Virus which makes no E1a at all

Design

Preclinical

Authors

MFMargaret FahnestockMcMaster UniversityJLJames B. LewisUniversity of Oxford

Discussion

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Implication

Hypothesis-generating for E1a domain mapping in viral models; leaves open translation to human adenovirus therapies.

Structured PICO

P
Population
Adenovirus type 2 E1a gene (pm975 variant) and infected cells
I
Intervention
Linker-scanning, deletion, and frameshift mutations in the E1a gene (specifically within the 46-amino-acid segment unique to the 289R protein)
C
Comparator
Virus which makes no E1a at all
O
Outcome
Ability to activate transcription of other viral early genes and endogenous hsp70 gene in vitro

The transactivating domain of the adenovirus E1a 289R protein contains specific structural components, including a putative metal-binding element and a beta-sheet region, important for transactivation function.

Cite This Study

Fahnestock et al. (1989) studied this question.

synapsesocial.com/papers/6a891fe59f05dd9930a2bbechttps://doi.org/10.1128/jvi.63.4.1495-1504.1989
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection.1983 · 290 citations
  2. 2Upstream regulatory regions required to stabilize binding to the TATA sequence in an adesovirus early promoter1987 · 57 citations
  3. 3E1A 13S and 12S mRNA Products Made in <i>Escherichia coli</i> Both Function as Nucleus-Localized Transcription Activators but Do Not Directly Bind DNA1985 · 188 citations
  4. 4An enhancer element is located 340 base pairs upstream from the adenovirus-2 E1A capsite1983 · 115 citations
  5. 5Isolation and Characterization of Full-Length cDNA Clones for Human α-, β-, and γ-Actin mRNAs: Skeletal but Not Cytoplasmic Actins Have an Amino-Terminal Cysteine that Is Subsequently Removed1983 · 1,211 citations