What question did this study set out to answer?

To define the functional contributions of the amino-terminal conserved region 1 of adenovirus serotype 5 E1a protein in cellular transformation and DNA synthesis.

September 1, 1988Molecular and Cellular Biology

The Amino-Terminal Region of the Adenovirus Serotype 5 E1a Protein Performs Two Separate Functions when Expressed in Primary Baby Rat Kidney Cells

View Full Paper

Ask AI

Bookmark

Authors

DSDeborah H. SmithMemorial Sloan Kettering Cancer Center EZEdward B. ZiffNYU Langone Health

Discussion

Member takes

Implication

Mutational analysis reveals two distinct E1a region 1 functions in primary rodent cells, indicating that cellular DNA synthesis induction is required for oncogenic transformation.

Key Points

To define the functional contributions of the amino-terminal conserved region 1 of adenovirus serotype 5 E1a protein in cellular transformation and DNA synthesis.
Engineered 14 recombinant adenovirus mutants bearing in-frame deletions and linker-insertions within or preceding conserved region 1 (amino acids 40 to 77).
Evaluated viral replication kinetics in HeLa cells alongside functional assays for focus formation and DNA synthesis induction in primary baby rat kidney cells.
All 14 mutant viruses displayed wild-type growth characteristics in HeLa cells, demonstrating that conserved region 1 is nonessential for viral replication in this cell type.
Mutational disruption revealed two genetically separable functions of region 1 in primary rodent cells: induction of cellular DNA synthesis and formation of transformed cell foci.
Impairment of the DNA synthesis induction function consistently correlated with a failure to induce focus formation in infected primary cells.

Cite This Study

Smith et al. (1988) studied this question.

synapsesocial.com/papers/6a83f69baddadcf8413c2199 https://doi.org/10.1128/mcb.8.9.3882-3890.1988

View Full Paper

Ask AI

Bookmark

Implication

Mutational analysis reveals two distinct E1a region 1 functions in primary rodent cells, indicating that cellular DNA synthesis induction is required for oncogenic transformation.

The Amino-Terminal Region of the Adenovirus Serotype 5 E1a Protein Performs Two Separate Functions when Expressed in Primary Baby Rat Kidney Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

The Amino-Terminal Region of the Adenovirus Serotype 5 E1a Protein Performs Two Separate Functions when Expressed in Primary Baby Rat Kidney Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider