The study identifies that reverse transcriptase-associated RNase H mediates the endonucleolytic release of the polyadenylate tail during retroviral reverse transcription.
May inform RNase H-targeted antivirals; leaves open translation to human retroviral disease.
By examining enzymatic reactions in vitro, we found that an early event during reverse transcription of the avian myeloblastosis virus RNA genome is the release of the 3' polyadenylate tail from the viral RNA template. By using specially constructed molecules containing minus-strand, strong-stop DNA and the 3' end of the viral RNA genome, we found that the reverse transcriptase-associated RNase H is responsible for the endonucleolytic release of polyadenylate.
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Olsen et al. (1985) studied this question.
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