February 1, 1979Journal of VirologyOpen Access

Reverse transcriptase from avian myeloblastosis virus

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Population

Avian myeloblastosis virus (lots of 20 to 30 g)

Design

Preclinical

Authors

Discussion

High-purity enzyme enables in vitro retroviral assays; leaves open extension to human applications.

Population

Avian myeloblastosis virus (lots of 20 to 30 g)

Intervention

Two-step column chromatographic procedure employing DEAE (DE 52) and carboxymethylcellulose (CM 52)

Outcome

Yield and purity of RNA-dependent DNA polymerasesurrogate

A two-step chromatographic procedure successfully purifies RNA-dependent DNA polymerase from avian myeloblastosis virus to >95% purity.

Houts et al. (1979) studied this question.

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