Key result
Formamide disruption of 60S AMV RNA to 30-40S RNA subunits increased template activity for viral reverse transcriptase by 2 to 3 times compared to 60S RNA.
Effect estimate: 2 to 3 times more active
Formamide disruption of AMV 60S RNA produces 30-40S subunits that retain a firmly bound 4S RNA resident primer, resulting in increased template activity for reverse transcriptase compared to the intact 60S RNA.
Controlled disruption of 60S AMV RNA with formamide was used to prepare 50-55S and 30-40S RNAS. When the activities of these RNAs as templates for AMV reverse transcriptase were compared it was found that 50-55S RNA was 1-5 times and 30-40S RNA 2 to 3 times more active than 60S RNA. The 30-40S RNA produced by heating, instead of formamide disruption, was inactive as a template but activity was restored by addition of oligo(dT). 40% of the 4S RNA initially associated with the 60S RNA remained associated with all the RNA species obtained by formamide treatment but was lost on heating. It is concluded that this RNA acts as resident primer whereas the other 60% of the 4S RNA is less firmly bound and appears to have little or no primer activity. Removal of the less firmly bound 4S RNA increases the template activity of the viral RNA.
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Trávníček et al. (1976) studied this question. Formamide disruption of 60S AMV RNA to prepare 50-55S and 30-40S RNAs vs. 60S RNA was evaluated on Template activity for AMV reverse transcriptase (2 to 3 times more active). Formamide disruption of 60S AMV RNA to 30-40S RNA subunits increased template activity for viral reverse transcriptase by 2 to 3 times compared to 60S RNA.
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