What question did this study set out to answer?

To investigate whether selectively inhibiting inducible nitric oxide synthase (iNOS) with L-NIL provides targeted anti-arthritic protection compared to non-selective NOS inhibition with L-NMMA in experimental arthritis.

February 1, 2001The Journal of Immunology

Selective Inhibition of Inducible Nitric Oxide Synthase Exacerbates Erosive Joint Disease

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Authors

NMNancy McCartney‐FrancisNational Institutes of Health DMDiane MizelNational Institutes of Health SWSharon M. WahlRoche (Switzerland)

Discussion

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Implication

Preclinical study demonstrates selective iNOS inhibition worsens joint destruction in arthritic rats, indicating critical roles for constitutive nitric oxide synthases.

Key Points

To investigate whether selectively inhibiting inducible nitric oxide synthase (iNOS) with L-NIL provides targeted anti-arthritic protection compared to non-selective NOS inhibition with L-NMMA in experimental arthritis.
Assessed in vitro nitrite production by streptococcal cell wall-stimulated rat mononuclear cells treated with L-NMMA or L-NIL.
Administered daily systemic L-NIL or L-NMMA to rats with streptococcal cell wall-induced arthritis to evaluate synovitis, joint swelling, and bone and cartilage loss.
Performed immunohistochemical analyses of arthritic synovium to evaluate the presence of iNOS, endothelial NOS (eNOS), and neuronal NOS (nNOS).
Unlike non-selective inhibition with L-NMMA which ablated synovitis, selective iNOS inhibition with L-NIL failed to reduce acute inflammation and exacerbated chronic disease, driving profound bone and cartilage loss.
Treatment with L-NIL reduced synovial iNOS-positive cell counts but left the distinct expression patterns of endothelial and neuronal NOS unaffected.

Cite This Study

McCartney‐Francis et al. (2001) studied this question.

synapsesocial.com/papers/6a895cfa75060194fdc97bf2 https://doi.org/10.4049/jimmunol.166.4.2734

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Implication

Preclinical study demonstrates selective iNOS inhibition worsens joint destruction in arthritic rats, indicating critical roles for constitutive nitric oxide synthases.

Selective Inhibition of Inducible Nitric Oxide Synthase Exacerbates Erosive Joint Disease

Discussion

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Implication

Key Points

Cite This Study

Selective Inhibition of Inducible Nitric Oxide Synthase Exacerbates Erosive Joint Disease

Discussion

Member takes

Implication

Key Points

Cite This Study

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