Key result
In rabbit portal vein smooth muscle cells, application of cyclic nucleotides and their dependent protein kinases (PK-A and PK-G) significantly inhibited L-type calcium current.
Population
Freshly isolated single smooth muscle cells from the rabbit portal vein
Design
Preclinical
Authors
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Provides mechanistic insight into vasodilators but remains preclinical; leaves open validation in human vessels.
In rabbit portal vein smooth muscle cells, cyclic nucleotide-dependent protein kinases (PK-A and PK-G) inhibit L-type calcium channels, providing a mechanism for the action of some vasodilators.
Xiong et al. (2008) studied this question. Cyclic nucleotides (8-Br-cAMP, 8-Br-cGMP) and protein kinases (PK-A, PK-G) was evaluated on L-type Ca2+ current (ICa(L)) peak amplitude. In rabbit portal vein smooth muscle cells, application of cyclic nucleotides and their dependent protein kinases (PK-A and PK-G) significantly inhibited L-type calcium current.
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