Demonstrates that specific poliovirus proteins accumulate in the host cell nucleus, potentially explaining the inhibitory activity of viral proteins on host transcription.
Answers are grounded in this paper and cite their sources.
May inhibit host transcription via nuclear protein accumulation; leaves open relevance to human pathogenesis or antiviral strategies.
Poliovirus (type 1, Mahoney) proteins were transported into the nuclei of HEp-2 cells, as demonstrated by means of electron microscopic autoradiography. Quantitative determinations of the proteins by electrophoresis showed that the relative amounts of precursor proteins (1a, 3b, 1b), of some of the intermediate polypeptides (3a, 3c, 5b, 2), and of one end product (VP1) were higher in the nucleus than in the cytoplasm. Other proteins (VPO, VP2, X, 4, 6a) were found in the same relative amounts in the nucleus as in the cytoplasm, whereas two proteins (VP3 and 6b) were excluded from the nucleus. The findings are discussed in view of the recently demonstrated inhibitory activity exerted by cytoplasmic extracts of poliovirus-infected cells on transcription in isolated nuclei of uninfected cells.
No takes yet. Share an insight, caveat, or question.
Bienz et al. (1982) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: