August 1, 2001Journal of Biological ChemistryOpen Access

Overexpression of Monomeric and Multimeric GIRK4 Subunits in Rat Atrial Myocytes Removes Fast Desensitization and Reduces Inward Rectification of Muscarinic K+ Current (IK(ACh))

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Population

Cultured atrial myocytes from adult rats, Chinese hamster ovary cells, and human embryonic kidney 293 cells

Comparison

Transient transfection of vectors encoding for… vs Transfection with a GIRK1 vector or baseline state

Design

Preclinical

Authors

KBKirsten BenderInstitute of Cell Biology MWMarie‐Cécile Wellner‐KienitzRuhr University Bochum AIAtsushi InanobeThe University of Osaka

Discussion

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Implication

Preclinical GIRK4 data should not yet change practice; leaves open subunit roles in human cardiac electrophysiology.

Structured PICO

Population

Cultured atrial myocytes from adult rats, Chinese hamster ovary (CHO) cells, and human embryonic kidney (HEK) 293 cells

Intervention

Transient transfection of vectors encoding for GIRK1 or GIRK4 subunits or GIRK4 concatemeric constructs (monomeric, dimeric, and tetrameric)

Comparator

Transfection with a GIRK1 vector (which did not cause any measurable effect) or baseline state

Outcome

Properties of macroscopic I(K(ACh)) including desensitization, inward rectification, and activation ratesurrogate

This basic science study demonstrates that homomeric GIRK4 complexes can form functional ion channels and that the kinetic properties of GIRK channels depend on their subunit composition.

Cite This Study

Bender et al. (2001) studied this question.

synapsesocial.com/papers/6a8985705edafe343d92e58d https://doi.org/10.1074/jbc.m102328200

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Overexpression of Monomeric and Multimeric GIRK4 Subunits in Rat Atrial Myocytes Removes Fast Desensitization and Reduces Inward Rectification of Muscarinic K+ Current (IK(ACh))

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Overexpression of Monomeric and Multimeric GIRK4 Subunits in Rat Atrial Myocytes Removes Fast Desensitization and Reduces Inward Rectification of Muscarinic K+ Current (IK(ACh))

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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