Adaptation of the Troponin T ELISA Test to a Microplate Immunoassay Reader

Surplus serum from patients in the cardiac care unit (n=42 for method comparison) and 63 healthy laboratory personnel.

Microtitre plate immunoassay method for measuring serum troponin T using Boehringer Mannheim reagents (30 microliters sample, 200 microliters reagents, 75 min duration).

ES-300 automatic analyser method (140 microliters sample, 700 microliters reagents, 90 min duration).

Correlation and agreement of serum troponin T concentrations between the two methods.surrogate