Key result
The encephalomyocarditis virus Leader protein binds directly to Ran-GTPase and inhibits nucleocytoplasmic transport, disrupting the RanGDP/GTP gradient and blocking nuclear export of mRNAs.
Population
HeLa cells, Xenopus egg extracts, and recombinant proteins (EMCV Leader protein, Ran-GTPase)
Comparison
Expression of EMCV Leader (L) protein vs Control
Design
Preclinical
Authors
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May illuminate picornaviral myocarditis mechanisms; leaves open Ran pathway as therapeutic target in humans.
The picornavirus Leader protein is the first viral picornaviral protein shown to interact directly with Ran and modulate the Ran-dependent nucleocytoplasmic pathway.
Porter et al. (2006) studied Encephalomyocarditis virus (EMCV) infection. EMCV Leader (L) protein vs. Control (GST alone or mutant L proteins) was evaluated on Binding to Ran-GTPase and inhibition of nucleocytoplasmic transport. The encephalomyocarditis virus Leader protein binds directly to Ran-GTPase and inhibits nucleocytoplasmic transport, disrupting the RanGDP/GTP gradient and blocking nuclear export of mRNAs.
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