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July 1, 1992Journal of VirologyOpen Access

Conservation of RNA-protein interactions among picornaviruses

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Key result

RNA-protein interactions between the 5' noncoding region of picornaviruses and cellular proteins are conserved among enteroviruses and cardioviruses, requiring specific stem-loop structures.

Population

HeLa cell extracts and other cell types used to study RNA-protein interactions with the 5' noncoding regions…

Design

Preclinical

Authors

SDSandra L. DildineUniversity of California, IrvineBSBert L. SemlerUniversity of California, Irvine

Discussion

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Implication

Indicates host factors suffice for picornavirus IRES activity; leaves open identification of specific mediators.

Structured PICO

P
Population
HeLa cell extracts and other cell types used to study RNA-protein interactions with the 5' noncoding regions of picornaviruses (poliovirus type 1, coxsackievirus type B3, human rhinovirus type 14, and Theiler's murine encephalomyelitis virus)
I
Intervention
Mutational analysis of stem-loop D (nucleotides 186 to 220) and stem-loop E (nucleotides 220 to 460) of the 5' NCR of poliovirus type 1
O
Outcome
RNA-protein interactions assessed by RNA mobility shifts assays

RNA-protein interactions involving the 5' noncoding regions of picornaviruses are conserved across different viral species, suggesting a basic function in cap-independent translation.

Cite This Study

Dildine et al. (1992) studied this question. Mutational analysis of 5' NCR stem-loop structures was evaluated on RNA-protein interactions between 5' NCR and cellular proteins. RNA-protein interactions between the 5' noncoding region of picornaviruses and cellular proteins are conserved among enteroviruses and cardioviruses, requiring specific stem-loop structures.

synapsesocial.com/papers/6a6c8be726a7f98052db42adhttps://doi.org/10.1128/jvi.66.7.4364-4376.1992
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Alterations in initiation factor activity from poliovirus-infected HeLa cells.1979 · 59 citations
  2. 2Determinants in the 5' noncoding region of poliovirus Sabin 1 RNA that influence the attenuation phenotype1989 · 233 citations
  3. 3Structural and functional analysis of the ribosome landing pad of poliovirus type 2: in vivo translation studies1991 · 186 citations
  4. 4Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.1989 · 115 citations
  5. 5A cellular 57 kDa protein binds to two regions of the internal translation initiation site of foot‐and‐mouth disease virus1990 · 66 citations