December 1, 1986AJP Cell Physiology
Calcium binding to cardiac sarcolemmal vesicles: potential role as a modifier of contraction
Population
Cardiac sarcolemmal vesicles isolated from rabbit ventricles
Comparison
Exposure to pharmacological agents, reduction of… vs Baseline/control ionic conditions
Design
Preclinical
Authors
Discussion
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Implication
Animal data question direct drug effects on sarcolemmal Ca binding; leaves open physiological modulation by Na, Mg, and depolarization in intact myocardium.
Structured PICO
P
Population
Cardiac sarcolemmal vesicles isolated from rabbit ventricles
I
Intervention
Exposure to pharmacological agents (ouabain, verapamil, nifedipine, Bay K 8644, caffeine, ryanodine, milrinone), reduction of extracellular [Na], and changes in membrane potential, Na, and Mg
C
Comparator
Baseline/control ionic conditions (e.g., [Na] = 140 mM, normal membrane potential)
O
Outcome
Changes in passive Ca binding at the external and internal sarcolemmal surfacessurrogate
Pharmacological agents like verapamil and ouabain do not alter external sarcolemmal Ca binding, but physiological changes in Na, Mg, and membrane potential significantly affect internal Ca binding, potentially modifying contraction.
Cite This Study
Bers et al. (1986) studied this question.