September 1, 1993The Journal of General PhysiologyOpen Access

Regulation of Ca2+ current in frog ventricular cardiomyocytes by guanosine 5'-triphosphate analogues and isoproterenol.

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Population

Frog ventricular cardiomyocytes

Comparison

Internal perfusion with hydrolysis-resistant GTP… vs Basal levels, washout conditions, and comparison…

Design

Preclinical

Authors

TPThomas D. ParsonsConference Board HHH. Criss HartzellElectrophysiology

Discussion

Member takes

Implication

Supports differential G-protein activation by hydrolysis-resistant analogues; hypothesis-generating for cardiac signaling, no clinical implications yet.

Structured PICO

Population

Frog ventricular cardiomyocytes

Intervention

Internal perfusion with hydrolysis-resistant GTP analogues (guanylyl 5'-imidodiphosphate [GppNHp] or guanosine 5'-thiotriphosphate [GTP gamma S]) and stimulation by isoproterenol (Iso) or forskolin (Forsk)

Comparator

Basal levels, washout conditions, and comparison between different GTP analogues

Outcome

Calcium currents (ICa) measured using the whole-cell patch clamp techniquesurrogate

Different hydrolysis-resistant GTP analogues behave differently in activating G proteins, demonstrating that the efficacy of G protein-effector interactions depends on the specific GTP analogue used.

Cite This Study

Parsons et al. (1993) studied this question.

synapsesocial.com/papers/6a89ca5a991508d05dc99aac https://doi.org/10.1085/jgp.102.3.525

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Implication

Supports differential G-protein activation by hydrolysis-resistant analogues; hypothesis-generating for cardiac signaling, no clinical implications yet.

Regulation of Ca2+ current in frog ventricular cardiomyocytes by guanosine 5'-triphosphate analogues and isoproterenol.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Regulation of Ca2+ current in frog ventricular cardiomyocytes by guanosine 5'-triphosphate analogues and isoproterenol.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider