Forty-two ejaculates from six different dairy bulls (five Holsteins and one Jersey) were used for this study. Following collection of each sample, three 1-ml. samples were taken from it for the experiment. These samples were treated as follows: No. 1, the diluting and cooling procedures were started immediately; no. 2, the semen was diluted immediately but the c001-
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Anderson et al. (1948) studied this question.
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