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April 29, 1997Proceedings of the National Academy of SciencesOpen Access

Thrombin cleaves recombinant human thrombopoietin: One of the proteolytic events that generates truncated forms of thrombopoietin

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Why the study?

Does thrombin cleave recombinant human thrombopoietin and modulate its biological activity?

Population

In vitro model using recombinant human thrombopoietin, human platelets from healthy volunteers, and purified…

Comparison

Incubation of rhTPO with thrombin or human… vs Incubation without thrombin, with thrombin…

Design

Preclinical

Authors

TKTakashi KatoVascular MedicineAOAtsushi OdaNational Agriculture and Food Research OrganizationYIYoshimasa InagakiKyowa Kirin (Japan)

Discussion

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Implication

Thrombin cleavage may regulate TPO activity; hypothesis-generating for in vivo platelet metabolism and leaves clinical relevance open.

Structured PICO

Does thrombin cleave recombinant human thrombopoietin and modulate its biological activity?

P
Population
In vitro model using recombinant human thrombopoietin (rhTPO), human platelets from healthy volunteers, and purified human plasma thrombin
I
Intervention
Incubation of rhTPO with thrombin or human platelets in the presence of calcium ions
C
Comparator
Incubation without thrombin, with thrombin inhibitor (hirudin), or without calcium ions
O
Outcome
Proteolytic cleavage of rhTPO and identification of cleavage sitessurrogate

Thrombin selectively cleaves human thrombopoietin at specific sites, generating truncated forms and modulating its biological activity, which may be important for platelet-related TPO metabolism.

Cite This Study

Kato et al. (1997) studied this question.

synapsesocial.com/papers/6a8a41960f137e402b337b4ahttps://doi.org/10.1073/pnas.94.9.4669
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular cloning and chromosomal localization of the human thrombopoietin gene1994 · 234 citations
  2. 2Thrombopoietin primes human platelet aggregation induced by shear stress and by multiple agonists1996 · 122 citations
  3. 3A sensitive sandwich ELISA for measuring thrombopoietin in human serum: serum thrombopoietin levels in healthy volunteers and in patients with haemopoietic disorders1996 · 207 citations
  4. 4The reciprocal relationship of thrombopoietin (c-Mpl ligand) to changes in the platelet mass during busulfan-induced thrombocytopenia in the rabbit1995 · 397 citations
  5. 5Erythropoietin structure-function relationships. Mutant proteins that test a model of tertiary structure1993 · 124 citations