What question did this study set out to answer?

To compare four blood processing techniques and identify the optimal method for detecting viremia in children with symptomatic enterovirus infections.

January 1, 1984Journal of Medical Virology

The isolation of enteroviruses from blood: A comparison of four processing methods

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Why the study?

Does the method of blood processing affect the isolation rate of enteroviruses in hospitalized children?

Population

28 children hospitalized with symptomatic enterovirus infections

Comparison

Blood processing methods for virus isolation vs Comparison among the four processing methods

Design

Cross-sectional

Authors

SPSandra L. PratherUniversity of Rochester Medical Center RDRon DaganBen-Gurion University of the Negev JJJerri Ann JenistaStrong Memorial Hospital

Discussion

Member takes

Implication

Blood processing method may affect enterovirus isolation in children; leaves open optimal diagnostic strategies pending prospective validation.

Key Points

To compare four blood processing techniques and identify the optimal method for detecting viremia in children with symptomatic enterovirus infections.
Analyzed blood samples from 28 children hospitalized with symptomatic enterovirus disease.
Tested four preparation methods for viral isolation: serum, mononuclear leukocytes, granulocytes, and plasma-mixed leukocytes.
Enteroviremia was confirmed overall in 11 of 28 children.
Mononuclear leukocytes yielded the highest isolation rate (9/11 children), followed by serum (7/11 children), granulocytes (3/10 children), and plasma-mixed leukocytes (3/11 children).
Mononuclear leukocytes provided the only positive blood isolate in four patients, while serum was the sole positive isolate in two patients.

Structured PICO

Does the method of blood processing affect the isolation rate of enteroviruses in hospitalized children?

Population

28 children hospitalized with symptomatic enterovirus infections

Intervention

Blood processing methods for virus isolation (method 1: serum, method 2: mononuclear leukocytes, method 3: granulocytes, method 4: plasma-mixed leukocytes)

Comparator

Comparison among the four processing methods

Outcome

Detection of enteroviremia (virus isolation rate)surrogate

Mononuclear leukocytes and serum are the most effective blood fractions for isolating enteroviruses in symptomatic children, indicating that processing methods significantly influence detection rates.

Cite This Study

Prather et al. (1984) studied this question.

synapsesocial.com/papers/6a8a4e9c1acf8e321fbd77ff https://doi.org/10.1002/jmv.1890140305

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Implication

Blood processing method may affect enterovirus isolation in children; leaves open optimal diagnostic strategies pending prospective validation.

The isolation of enteroviruses from blood: A comparison of four processing methods

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The isolation of enteroviruses from blood: A comparison of four processing methods

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider