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November 1, 1999Journal of Biological ChemistryOpen Access

Mapping of the Physical Interaction between the Intracellular Domains of an Inwardly Rectifying Potassium Channel, Kir6.2

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Population

In vitro protein-protein interaction assay of inwardly rectifying potassium channel subunits

Comparison

Mutation within the conserved region of the… vs Wild-type Kir channels

Design

Preclinical

Authors

STStephen J. TuckerBoston UniversityFAFrances M. AshcroftUniversity of Oxford

Discussion

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Overview

Kir6.2 domain interaction is required for channel function in vitro; leaves open relevance to human channelopathies pending in vivo validation.

Structured PICO

P
Population
In vitro protein-protein interaction assay of inwardly rectifying potassium (Kir) channel subunits (Kir6.2, Kir6.1, Kir2.1)
I
Intervention
Mutation within the conserved region of the N-terminal interaction domain
C
Comparator
Wild-type Kir channels
O
Outcome
Physical interaction between the N- and C-terminal intracellular domains and ability to form functional channelssurrogate

The physical interaction between the N- and C-terminal domains of Kir channels is highly conserved and essential for the formation of functional potassium channels.

Cite This Study

Tucker et al. (1999) studied this question.

synapsesocial.com/papers/6a8a588b755d28965dfd65d7https://doi.org/10.1074/jbc.274.47.33393
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular Determinants for Assembly of G-protein-activated Inwardly Rectifying K+ Channels1997 · 27 citations
  2. 2Identification of Regions That Regulate the Expression and Activity of G Protein‐Gated Inward Rectifier K<sup>+</sup> Channels in <i>Xenopus</i> Oocytes1997 · 25 citations
  3. 3ATP inhibition of K<sub>ATP</sub> channels: control of nucleotide sensitivity by the N‐terminal domain of the Kir6.2 subunit1999 · 96 citations
  4. 4Direct Photoaffinity Labeling of the Kir6.2 Subunit of the ATP-sensitive K+ Channel by 8-Azido-ATP1999 · 102 citations
  5. 5Combining evolutionary information and neural networks to predict protein secondary structure1994 · 1,462 citations