September 1, 1985The Journal of Cell BiologyOpen Access

Axial arrangement of the myosin rod in vertebrate thick filaments: immunoelectron microscopy with a monoclonal antibody to light meromyosin.

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Population

Chicken pectoralis myofibrils and purified myosin (vertebrate striated muscle model)

Design

Preclinical

Authors

TST. ShimizuKitasato University Medical Center JDJames E. DennisBaylor College of Medicine ΤMΤοmoh MasakiUniversity of Tsukuba

Discussion

Member takes

Implication

Supports Huxley's myosin packing model in animal sarcomeres; leaves open relevance to human cardiac contractility.

Structured PICO

Population

Chicken pectoralis myofibrils and purified myosin (vertebrate striated muscle model)

Intervention

Immunoelectron microscopy and immunofluorescence microscopy using monoclonal antibody MF20

Outcome

Axial arrangement and packing of light meromyosin (LMM) along the backbone of the thick filament

This study provides direct evidence that the 14-15-nm axial translation of light meromyosin in the thick filament backbone corresponds to the cross-bridge repeat, supporting Huxley's model of myosin packing.

Cite This Study

Shimizu et al. (1985) studied this question.

synapsesocial.com/papers/6a8a9ff7af6b08ee8d72472a https://doi.org/10.1083/jcb.101.3.1115

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Axial arrangement of the myosin rod in vertebrate thick filaments: immunoelectron microscopy with a monoclonal antibody to light meromyosin.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Axial arrangement of the myosin rod in vertebrate thick filaments: immunoelectron microscopy with a monoclonal antibody to light meromyosin.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider