March 1, 1976Journal of Applied Physiology
Reference sample method for cardiac output and regional blood flow determinations in the rat
Why the study?
Can the reference sample method be applied to smaller mammals for determinations of regional flows and cardiac output?
Population
Conscious rats
Comparison
Reference sample method using strontium-85… vs Previously reported values in rats using larger…
Design
Preclinical
Authors
Discussion
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Implication
Supports microsphere reference sampling in conscious rats; extends prior techniques but leaves open clinical translation.
Key Points
- To evaluate the reference sample method using radioactive microspheres for simultaneous determination of cardiac output and regional organ blood flows in conscious rats.
- Injected strontium-85-labeled microspheres (15 ± 5 µm in diameter) into the left ventricle of conscious rats.
- Withdrew reference blood samples of known volume from peripheral arteries to calculate absolute regional flow per gram of tissue.
- Measured cardiac output values agreed with previously reported physiological values in rats.
- Percentage distribution of cardiac output to the brain, lungs, and intestinal bed differed from earlier studies using larger microspheres.
Structured PICO
Can the reference sample method be applied to smaller mammals for determinations of regional flows and cardiac output?
P
Population
Conscious rats
I
Intervention
Reference sample method using strontium-85 labeled microspheres (15 +/- 5 mum in diam) injected into the left ventricle
C
Comparator
Previously reported values in rats using larger spheres
O
Outcome
Cardiac output and regional blood flowssurrogate
The reference sample method using small microspheres can be successfully applied to small mammals like rats for determining cardiac output and regional blood flow.
Cite This Study
Malik et al. (1976) studied this question.