Why the study?
Does solid-phase radioimmunoassay (RIA) improve the detection and characterization of measles IgG antibodies compared to hemagglutination inhibition (HI) in patients with SSPE and multiple sclerosis?
Does solid-phase radioimmunoassay (RIA) improve the detection and characterization of measles IgG antibodies compared to hemagglutination inhibition (HI) in patients with SSPE and multiple sclerosis?
A newly developed solid-phase RIA is highly sensitive for detecting measles IgG antibodies and reveals characteristically high RIA/HI antibody ratios in patients with SSPE, but not in those with multiple sclerosis.
A highly sensitive procedure of solid-phase radioimmunoassay (RIA) was developed for the detection of measles IgG antibody. HeLa cells persistently infected with measles virus were used as a solid-phase antigen. This technique was applied to the detection of measles IgG antibody in patients with subacute sclerosing panencephalitis (SSPE) and multiple sclerosis. Normal subjects having experienced natural measles or measles vaccination and patients with various neurological diseases of non-virus nature were also examined as control groups. Measles antibody was detected at high titers in both the sera and cerebrospinal fluid of SSPE patients. Moreover, RIA/HI ratios of SSPE patients were significantly higher than those of normal subjects, suggesting the presence in the formers of antibodies to nucleocapsids at high titers as well as to viral envelopes. On the other hand, no significant difference was found in both RIA and HI titers between the sera of multiple sclerosis and those of various neurological diseases.
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Sakata et al. (1979) studied this question.
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